Initiate the license verification by calling verifyLicense(). This method is exposed in the LicensingService class. It takes two parameters as input: Zhong, X. et al. DOMAINS REARRANGED METHYLTRANSFERASE3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis. Proc. Natl Acad. Sci. USA 112, 911–916 (2015). Hossain, M. S. et al. Divergent cytosine DNA methylation patterns in single-cell, soybean root hairs. N. Phytol. 214, 808–819 (2017).

Marfil, C. et al. Changes in grapevine DNA methylation and polyphenols content induced by solar ultraviolet-B radiation, water deficit and abscisic acid spray treatments. Plant Physiol. Biochem. 135, 287–294 (2019). Kaiserli, E. & Jenkins, G. I. UV-B promotes rapid nuclear translocation of the Arabidopsis UV-B specific signaling component UVR8 and activates its function in the nucleus. Plant Cell 19, 2662–2673 (2007).A) In vitro methylation assay of DRM2 or C397R on DNA with different sequence contexts. CG, (GAC) 12; CTA, (TAC) 12; CAA, (AAC) 12; CTG, (TGC) 12. Data are means ± SD ( n = 3 replicates). Statistical analysis used two-tailed Student’s t test for the difference from WT: * P< 0.05, *** P< 0.001, and **** P< 0.0001. ( B) Ribbon representation of DRM2 C397R bound to CCG DNA and SAH. Hydrogen-bonding interactions formed between the side chain of R397 and G12 are depicted as dashed lines in expanded view. The bases of C11 and G12 in the expanded view are colored yellow. ( C) Metaplots showing average methylation level of DRM2, C397R, and ddc over TEs for CG, CHG, and CHH contexts. ( D) Representative genomic regions of two TEs on chromosome 3 (AT3TE666360 and AT3TE28430/AT3TE28440) showing the methylation levels of Col-0, ddc, DRM2/ ddc, and C397R/ ddc. ( E) Bar chart showing the total number of DMCs in each context of DRM2/ ddc and C397R/ ddc called against ddc. ( F) Motif of the 4 nucleotides upstream and 5 nucleotides downstream of hyper DMCs in C397R called against ddc ( n = 29,347). Huang L, Jones AM, Searle I, Patel K, Vogler H, et al. (2009) An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II. Nat Struct Mol Biol 16: 91–93. Yang, Y. et al. UVR8 interacts with WRKY36 to regulate HY5 transcription and hypocotyl elongation in Arabidopsis. Nat. Plants 4, 98–107 (2018).

Figure 1. A conserved clade of catalytically mutated DRM cytosine methyltransferase paralogs in angiosperms. Zientara-Rytter, K. & Sirko, A. Significant role of PB1 and UBA domains in multimerization of Joka2, a selective autophagy cargo receptor from tobacco. Front. Plant Sci. 5, 13 (2014). Zhou, M., Sng, N. J., LeFrois, C. E., Paul, A.-L. L. & Ferl, R. J. Epigenomics in an extraterrestrial environment: organ-specific alteration of DNA methylation and gene expression elicited by spaceflight in Arabidopsis thaliana. BMC Genomics 20, 205 (2019). All necessary IAM Roles and Policies needed to make the solution work, following least privilege principles.We first tested whether the catalytic and UBA mutations introduced into DRM2 had any consequence on protein accumulation and localization. Intact DRM2-Myc drm1 drm2 nuclei immunostained using α-Myc antibodies show signal throughout the nuclei with numerous bright foci and exclusion from the strongly DAPI-staining chromocentres ( Figure 4B) [53]. The DRM2 cat-Myc and DRM2 uba-Myc mutant proteins showed comparable staining patterns to those observed for the unmutated DRM2-Myc protein ( Figure 4B). This indicates that the UBA and methyltransferase mutations introduced do not cause a defect in DRM2 protein stability or localization to the nucleus. Slotkin, R. K. & Martienssen, R. Transposable elements and the epigenetic regulation of the genome. Nat. Rev. Genet. 8, 272–285 (2007).

Law JA, Jacobsen SE (2010) Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet 11: 204–220. Tavridou, E., Pireyre, M. & Ulm, R. Degradation of the transcription factors PIF4 and PIF5 under UV-B promotes UVR8-mediated inhibition of hypocotyl growth in Arabidopsis. Plant J. 101, 507–517 (2020). Trapnell, C. et al. Transcript assembly and quantification by RNA-seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat. Biotechnol. 28, 511–515 (2010).Heijde, M. et al. Constitutively active UVR8 photoreceptor variant in Arabidopsis. Proc. Natl Acad. Sci. USA 110, 20326–20331 (2013). A connection between the corporate on-premises environment and AWS through VPN or AWS Direct Connect.

Henderson IR, Zhang X, Lu C, Johnson L, Meyers BC, et al. (2006) Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning. Nat Genet 38: 721–725. Zemach, A. et al. The Arabidopsis nucleosome remodeler DDM1 allows DNA methyltransferases to access H1-containing heterochromatin. Cell 153, 193–205 (2013).Kaneda M, Okano M, Hata K, Sado T, Tsujimoto N, et al. (2004) Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature 429: 900–903. Xi, Y. & Li, W. BSMAP: whole genome bisulfite sequence MAPping program. BMC Bioinform. 10, 232 (2009). Aravin AA, Bourc'his D (2008) Small RNA guides for de novo DNA methylation in mammalian germ cells. Genes Dev 22: 970–975.