Rosalina has held a grudge against her since Jeffy's Parents! She can't stand her beating up Jeffy. When Jeffy gets taken away, she got frustrated with Mario, yelling that she can't believe he let Nancy take him away, trying to convince him that Nancy beats Jeffy, but Mario shows absolutely no concern, even stating that it's good that she beats him, leading Rosalina to break up with Mario for his heartlessness, albeit temporarily. She resents the woman.

There have been times where Mario would argue, but these arguments mostly have been caused by Jeffy. It was claimed by Chilly in her Facebook that that the real reason why Rosalina hadn't appeared for a while was that her voice actress Elaina no longer works in SML, hence that she attends an out of state college far away from Florida. However, she has officially returned in Doctor Jeffy! proving she still exists and has seemed to have returned as an on and off main character.She can get defensive of Jeffy to the point that she ruined her own husband's reputation, claiming to the police that Mario likes to beat Jeffy up all the time, which is not factual. Rosalina's ex-boyfriend that Mario got jealous over. D-Money broke up with her since she was being dragged along with him too much. Her parents had preferred her dating him instead of Mario.

Another possibility is that the videos where she is vegan are set in the human puppet universe while the main universe Rosalina has a normal diet.She is the only member of Mario's current family not to have met Luigi depending on if the Luigi's Mansion series is canon. In Mario's New Hat!, the two's wedding day has finally arrived. Though she almost calls it off when Mario mysteriously disappears. Once Mario finally shows up as a Frog and explains what happened, Rosalina declares that she doesn't care what he looks like, and she loves him for him. Goodman (after comically warning Mario that she'll take everything he owns because she didn't sign the prenup) makes them take their vows and they finally get married, with their kiss breaking the spell over Mario. Rosalina first met Bowser Junior in Jeffy's Birthday!, when Jeffy was about to go on the jungle gym with Junior and his friends, and Junior found her really hot and liked her breasts. In Jeffy's Cellphone!, Rosalina was with Mario the whole day, leaving there no time for Mario to call a prostitute. She also attempted to call the police on Mario in The Dead Body!, assuming that Mario purposely killed a hobo, without any evidence that he purposely did so, or letting him give an explanation.

There are the Sparks themselves, which resemble the star-like Lumas who once assisted Mario on his adventures. And then there’s the host of varied worlds that make up this new game’s interplanetary playspace: Santorini-style Greek islands, strange coral cliff faces, blizzard-blasted mountaintops, and autumnal forests home to giant spongy pumpkins. New Kirkhope Yet Rabbid Rosalina is also aspirational. Despite her fatigue, she’s still showing up – and getting the job done better than any of her opponents. “When she's called to action, she just reacts like, ‘OK, must I, sure, do I have to?’,” Nava says. “But then, when she's on the battlefield, she’s really super powerful. She’s brave. And she’s a fighter.” Post Jeffy's Bad Word, she only abuses Mario whenever he doesn’t stay out of her way or when he abuses Jeffy. Since Logan is now family friendly, Rosalina doesn’t abuse Mario anymore as of now, she just sometimes yells at him when he acts greedy or cruel. Rosalina and Shrek dated in Shrek's Coma!, showing that Rosalina likes Shrek and considered him a nice guy. However, they broke up in the same episode. Their puppets are now released on smlmerch.com. Also, Rosalina's puppet design has slightly changed from the Meet the Cast design meaning the one shown in the vlog was a prototype.As of 2020, she acts more antagonistic and her personality has become more whiny, cruel, insensitive, unintelligent, cranky, and spoiled. She is also incredibly hypocritical, as she openly flirts with her many ex-boyfriends and dismisses Mario's complaints as him being jealous, while constantly accusing Mario of cheating on her and once trying to kill him just for texting Peach. Her hypocritical side is also shown in " Goodman's New Life!," where she verbally abuses and puts down her husband so many times, but when Marvin (unknowingly to her Goodman) called her fat once, she decides to file for divorce. Her overall disposition also implies that she has come to hate Mario for not catering to her whims and may only be staying with him for Jeffy's sake (or possibly his money). In Smart Jeffy, Junior caught Rosalina's attention by calling her "Busty Tits" and Junior explaining to her what happened to Jeffy. Due to the fact she was attracted to Atso's body in " Shrek's Coma!" she is show to have pedophilia urges Big Red • Kung Pow • Baby Peach • Taco Bell Chihuahua • Mama Luigi • Speedy • Pimpster Goomba • Skittles • Shrek Junior • Taliban • Blue • Chad • King Pig • Ray the Pig • Helmet Pig • Yoshi • Koopa Troopa • Atso • Brown Elephant • Lazlo • Mr. Pig • Peach • Tony the Tiger • Woody • Mr. Magoo • Luigi • Donkey Kong The only time Rosalina gets frustrated with Jeffy was in Jeffy Gets Potty Trained! when she forced him to eat his peas, but Jeffy demanded marshmallows instead. She also sometimes gets mad at him in recent videos. And in Jeffy's first apperance ( Mario the Babysitter!) she did not like Jeffy and was even a little scared of him.