After the membranes were blocked with 5% skim milk in Tris-buffered saline (TBS) for at least 1 h, they were incubated with the indicated antibodies overnight, washed with TBS three times, and then incubated with horseradish peroxidase-conjugated secondary antibodies for 2 h. mg/ml) clinical study, AR68 was applied three times per week for 24 weeks, and through quantitative analysis using a phototrichogram, we confirmed increases in total hair counts. Subjects were selected on the basis of inclusion and exclusion criteria and consisted of 48 Korean males and females ranging from 22 to 53 years of age. However, it is doing so via treatment with self-assembled micelle inhibitory RNA (SAMiRNA) nanoparticle-type siRNA. If you only test a product for a year or two and you don’t see any regrowth, it’s almost impossible to say whether it stops hair loss.

If any adverse reaction occurred, the dermatologist’s assessment of the adverse reaction was further conducted in accordance with the standard operating procedures for adverse reactions. During the study period, six subjects withdrew (withdrawal of consent or loss to follow-up), ten subjects withdrew at the investigator's discretion (hair perm, dyeing, etc.If there is something which promises to be a hair loss cure, there must be clear results to convince me.

In this study, treatment with self-assembled micelle inhibitory RNA (SAMiRNA) nanoparticle-type siRNA selectively suppressed AR expression in vitro. This microRNA could aid hair regrowth by softening hair follicles, which naturally become stiffer as we age and contribute to hair loss. To increase efficacy and user convenience, we designed and conducted a clinical study with high-dose (5 mg/ml) treatment once per week.The presence of blockbuster products like Humira for autoimmune treatments indicates the significance of finding effective solutions for conditions such as rheumatoid arthritis, intestinal disease, and psoriasis. Once the siRNA molecules are delivered to the hair follicle cells, they bind to complementary mRNA molecules and trigger RNA interference. For evaluation of total hair counts, the hair was clipped approximately 2 mm after a red spot tattoo in the evaluation area (1 cm 2) of the hair loss region (forehead hairline or vertex). Based on the essential role of AR in AGA pathogenesis, suppression of AR expression may be an ideal approach for AGA treatment. AR68 treatment significantly reduced the AR mRNA and protein levels in HFDPCs and human hair follicles.