To use it as a deodorant, simply apply it under your arms and anywhere else that is prone to sweat and body odor. Additionally, ozonated olive oil should be stored in dark glass bottles to prevent degradation from light exposure.(3) According to one paper, it “can be stored for around a year at room temperature or up to three or four years in a refrigerator.”(5) The activated oxygen is then funnelled into the desired raw oil where the electrically charged oxygen is slowly infused. The result: a creamy oil that is reported to do wonders for your skin. Ozone produced by O3 generators is so safe that its used the world over in operating rooms, the organic food industry, swimming pools, municipal water systems, and many more. And limited research seems to point towards ozonated oils (like sunflower seed oil) being able to eliminate both Gram-positive and Gram-negative bacteria in Petri dishes (not on human beings).(7) And ozonated sunflower seed oil appears to have anti-fungal properties too!(5)

A study published in 2012 investigated the use of ozonated olive oil to treat certain periodontal conditions in 20 subjects. They found that when ozonated olive oil was used as an adjunctive treatment to root planning or scaling it resulted in significant improvements for the participants who used it. (1) Cytotoxicity was evaluated quantitatively by a reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. First, 1 × 10 4 cells in the logarithmic growth phase were seeded in each well of a 96-well plate in complete culture medium and incubated overnight (37 °C, 5% CO 2). Next, the medium was aspirated, and tested oils were added to achieve final concentrations from 39 to 1250 µg mL −1. The negative control contained cells in DMEM. Cells were exposed to extracts for 24 h (37 °C, 5% CO 2). After incubation, the samples were aspirated, MTT (0.5 mg mL −1 in PBS) was added, and they were incubated for further 3 h. Next, it was removed and formazan precipitates were solubilised by DMSO. Absorbance was measured at 550 nm (with a reference filter of 620 nm) using a microplate reader (TriStar 2 LB 942, Berthold Technologies GmbH & Co. KG, Germany). Because “olive oil has also proven to have antimicrobial activity in infections of burnt skin, against Gram-positive bacteria, such as Staphylococcus aureus, Gram-negative bacteria, and various species of fungi, including Candida spp… [and] it has been demonstrated that it is possible to further enhance the properties of oils by adding ozone.”(5) Because of its antioxidant actions, ozonated olive oil can also be used to eradicate some of the signs of aging like wrinkles and diminish the appearance of age spots. Other Uses for the Skin include: Battinelli L, Daniele C, Cristiani M, Bisignano G, Saija A, Mazzanti G. In vitro antifungal and anti-elastaseactivity of some aliphatic aldehydes from Olea europaea L. fruit. Phytomedicine. 2006; 13:558-563

Ozonated Olive Oil for the Teeth

Barrientos S, Brem H, Stojadinovic O, Tomic-Canic M. Clinical application of growth factors and cytokines in wound. Wound Repair and Regeneration. 2014; 22(5):569-578. DOI: 10.1111/wrr.12205 Ozonated Olive Oil is a powerful natural remedy for a variety of health concerns, especially those related to skin health. I wanted to share an overview of just exactly what this healing oil is, and how it works. What Is Ozonated Olive Oil? Fabiani R, Rosignoli P, De Bartolomeo A, Fuccelli R, Servili M, Montedoro GF, Morozzi G. Oxidative DNA damage is prevented by extracts of olive oil, hydroxytyrosol, and other olive phenolic compounds in human blood mononuclear cells and HL60 cells. Journal of Nutrition. 2008; 138:1411-1416 Ozonated olive oil is made with high quality, organic, extra-virgin, cold-pressed olive oil that is put through a process of "ozone injection", which is bubbling ozone into the liquid for an extended period of time. Many companies use a cheap corona discharge ozone generator that can lead to contamination. For purity and quality, a cold plasma ozone generator should always be used. When searching for a high-quality source of ozonated olive oil, ask the company if they are using a corona or cold plasma unit. Ozone is a reactive gas and is very hard to stabilize, but by passing it through the oil, contained within an ozone resistant container, the ozone gas begins to interact and stabilize within the oil. As it is continually processed, the ozone slowly begins to turn the liquid form of the oil into an off-white colored paste. Ozonated Olive Oil Liquid Vs. Ozonated Olive Oil Paste

Elshinawy, M., Al-Madboly, L.A., Ghoneim, W.M., & El-Deeb, N.M. (2018). Synergistic effect of newly introduced root canal medicaments; ozonated olive oil and chitosan nanoparticles, against persistent endodontic pathogens. Frontiers in Microbiology, 9, 1371. doi: 10.3389/fmicb.2018.01371 Due to their antibacterial properties, ozonated oils have been used for irritated skin, small cuts and scratches and pimples. You can also try it on mature skin to reduce the signs of aging. Below are a few uses for ozonated oils. Ozonated olive oil is most commonly used for the skin. It can be applied directly to the skin in exactly the same way that you would use a skin cream or salve.It is produced by using very high quality, cold pressed virgin olive oil which is put through a manufacturing process referred to as ‘ozone injection’. This process involves bubbling the ozone into the oil for a fairly extended period until it is ready for use. Georgiev, V. F., Batakliev, T. T., Anachkov, M. P., & Rakovski, S. K. (2015). Study of Ozonated Olive Oil: Monitoring of the Ozone Absorption and Analysis of the Obtained Functional Groups. Ozone: Science and Engineering, 37(1), 55–61. httpss://doi.org/10.1080/01919512.2014.904736