Sohu (in Chinese). May 10, 2013. Archived from the original on March 24, 2020 . Retrieved February 26, 2017.

Finally, we attempted to identify the proteins targeted by SlMPK20 by screening a tomato cDNA library using SlMPK20 as the bait through Y2H assay. We obtained 89 proteins that interacted with SlMPK20. Because MAPKs generally regulate transcription factors at the post-translational level, we focused on four transcription factors among the 89 proteins interacting with SlMPK20. These include Solyc06g083980.1.1 (BHLH transcription factor), Solyc05g009880.2.1 (Transcription factor bHLH30), Solyc11g069340.1.1 (Zinc finger transcription factor 69, SlC3H69) and Solyc10g055410.1.1 (R2R3MYB transcription factor 32, SlMYB32). Among them, SlMYB32 shares a 56% identity with its ortholog, AtMYB32 (Fig. S12). Because AtMYB32 was involved in Arabidopsis pollen development (Preston etal., 2004), we selected SlMYB32 for further analyses.Fresh stamens were snap-frozen in liquid nitrogen. The sugar concentrations were measured by HPLC (Agilent 1100, Santa Clara, CA, USA) using the respective sucrose, d-(−)-fructose and d-(+)-glucose (Sigma) as standards (Methods S5). Measurement of endogenous IAA and JA concentrations Crystal Liu (born An Feng; August 25, 1987), [3] better known by her stage name Liu Yifei ( Chinese: 刘亦菲; pinyin: Liú Yìfēi), is a Chinese-American [a] actress, singer, and model. [6] She has appeared multiple times on Forbes China Celebrity 100 list and was named one of the New Four Dan actresses of China in 2009. [7] She is widely known as "Fairy Sister" in China. [8] [9]

Liu Yifei's New Drama 'Rose Story' Officially Announced, First Poster Revealed | TimeSparker - Movie and TvShow pop culture, news, release information". TimeSparker . Retrieved July 23, 2023.With our approach, we were able to provide insights into CS functions in tumor immunity. First, we revealed the relationships between senescence levels and immune features in individual cancer types, deepening our understanding of the exact roles of CS in context-dependent mediation [ 70]. Second, CS scores showed positive correlations with total cell infiltration levels and PD-L1 expression in particular cancer types, including melanoma and prostate cancer. These prominent associations may provide possible explanations for age-dependent immune dysfunctions in these two cancer types [ 71, 72]. Third, we observed that senescent cancer cells from PRAD patients exhibited activated immune properties, showing increased expression of HLA genes and frequent communication with T-cells. These results suggest potential mechanisms underlying previous observations that immune surveillance of senescent cells enhanced immune clearance and limited tumor progression [ 70]. Further investigations based on the assessment of CS levels would facilitate mechanistic understanding at the systems level in more age-related diseases. In Situ hybridization was performed as described previously (Wang & Ruan, 2016). Templates for the SlMPK20-specific probes were generated using primer pairs: Yw-SlMPK20-F/Yw-SlMPK20-R (Table S1). Regan SM, Moffatt BA. 1990. Cytochemical analysis of pollen development in wild-type Arabidopsis and a male-sterile mutant. Plant Cell 2: 877– 889. We then performed Western blot analyses on proteins extracted from the stamens of WT and five CR lines at binucleate stage using the anti-SlMPK20 antibody (Fig. S1b). The analysis detected the SlMPK20 in the WT samples but not in the CR lines (Fig. 2c), confirming the absence of SlMPK20 in the transgenic plants.

Tabata R, Ikezaki M, Fujibe T, Aida M, Tian CE, Ueno Y, Yamamoto KT, Machida Y, Nakamura K, Ishiguro S. 2010. Arabidopsis auxin response factor6 and 8 regulate jasmonic acid biosynthesis and floral organ development via repression of class 1 KNOX genes. Plant Cell Physiology 51: 164. a b "美了多年的刘亦菲一直是那个神仙姐姐". Sina (in Chinese). March 4, 2016. Archived from the original on May 15, 2020 . Retrieved February 26, 2017.Total protein was extracted from stamens at the specified stages with two biological replicates. The protein samples ( c. 100μg each) were digested with trypsin. The resultant peptides were labeled using the 6-plex TMT kit (Thermo, Waltham, MA, USA). The TMT-labeled samples were combined and then fractionated by high pH reverse-phase HPLC using Agilent 300 Extend C18 column (5μm particles, 4.6mm i.d., 250mm length). Quantitative proteomic analyses were conducted using LC-MS/MS as described in detail in Methods S4 based on Guo etal. ( 2017). Measurement of sucrose, fructose and glucose contents Wang Y, Zhang WZ, Song LF, Zou JJ, Su Z, Wu WH. 2008. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiology 148: 1201– 1211. In 2022, she won the Best Actress Award at the 13th Macau International Television Festival for her role as Zhao Paner in the costume drama "Meng Hua Lu"(梦华录). Pan CT, Ye L, Qi L, Liu X, He YJ, Wang J, Chen LF, Lu G. 2016. CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations. Scientific Reports 6: 24765. The older you get, the greater your chance of developing health problems. So taking out life insurance when you’re young and healthy could cost less than starting a policy in later life. 2. Stay healthy