Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Thung Phaya, Ratchathewi, Bangkok, 10400, Thailand

R. Komura, T. Kawakami, K. Nakajima, H. Suzuki and C. Nakashima, J. Gen. Plant Pathol., 2018, 84, 247–253 CrossRef CAS. It’s pretty easy to design a minion bedroom for twins as well, although it’s double the cost of one!But in case you’re open to much more variety, then HERE are the curtains that can help you create your minion bedroom so damn easily! Don’t Forget The Lamp Light S. A. Bustin, V. Benes, J. A. Garson, J. Hellemans, J. Huggett, M. Kubista, R. Mueller, T. Nolan, M. W. Pfaffl, G. L. Shipley, J. Vandesompele and C. T. Wittwer, Clin. Chem., 2009, 55, 611–622 CrossRef CAS PubMed. W. Sun, P. Qin, H. Gao, G. Li and K. Jiao, Biosens. Bioelectron., 2010, 25, 1264–1270 CrossRef CAS PubMed. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Publisher’s Note P. K. Drain, E. P. Hyle, F. Noubary, K. A. Freedberg, D. Wilson, W. R. Bishai, W. Rodriguez and I. V. Bassett, Lancet Infect. Dis., 2014, 14, 239–249 CrossRef PubMed.

The above one is a cute minion printable you might love getting framed and decorating your bedroom with. Get this cute wall art printable HERE . A Lot Of Minion Figurines Are Classy! Image Source L. Becherer, S. Knauf, M. Marks, S. Lueert, S. Frischmann, N. Borst, F. von Stetten, S. Bieb, Y. Adu-Sarkodie, K. Asiedu, O. Mitja and M. Bakheit, Emerging Infect. Dis., 2020, 26, 282–288 Search PubMed. P.-H. Chou, Y.-C. Lin, P.-H. Teng, C.-L. Chen and P.-Y. Lee, J. Virol. Methods, 2011, 173, 67–74 CrossRef CAS PubMed. Dondorp AM, Nosten F, Yi P, Das D, Phyo AP, Tarning J, et al. Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2009;361:455–67. Your kids will love it when, at night, even when everything seems dark and ijn=visible, the minions appear when the lamp light goes on! Sounds cool? You can buy this minion lamp HERE. How About A Minion Rug?The recent spread of artemisinin (ART)-resistant Plasmodium falciparum represents an emerging global threat to public health. In Southeast Asia, the C580Y mutation of kelch13 ( k13) is the dominant mutation of ART-resistant P. falciparum. Therefore, a simple method for the detection of C580Y mutation is urgently needed to enable widespread routine surveillance in the field. The aim of this study is to develop a new diagnostic procedure for the C580Y mutation using loop-mediated isothermal amplification (LAMP) combined with the MinION nanopore sequencer. Results Describes the repeatability (intraassay variance) and reproducibility (interassay variance) of an NAAT 53 Y. Zhou, J. Xiao, X. Ma, Q. Wang and Y. Zhang, Appl. Microbiol. Biotechnol., 2018, 102, 5299–5308 CrossRef CAS PubMed. Clinical Laboratory Improvement Amendments (CLIA), available at: https://www.cms.gov/Regulations-and-Guidance/Legislation/CLIA/index.html. B. Tian, Z. Qiu, J. Ma, T. La Zardán Gómez de Torre, C. Johansson, P. Svedlindh and M. Strömberg, Biosens. Bioelectron., 2016, 86, 420–425 CrossRef CAS PubMed.

This work was supported in part by the National BioResource Project in Japan. ( http://www.nbrp.jp/). Competing interests N. Nakamura, K. Ito, M. Takahashi, S. Hongo, K. Hashimoto, M. Kawamoto, A. Taniguchi, N. Kamatani and N. Gemma, Clin. Biochem., 2009, 42, 1158–1161 CrossRef CAS. Want to add this crazy little green minion to your collection? This cute little minion figurine is HERE. Add A Few Movie Moments… Cheng Q, Cunningham J, Gatton ML. Systematic review of sub-microscopic P. vivax infections: prevalence and determining factors. PLoS Negl Trop Dis. 2015;9:e3413. Division of Biomedical Sciences, Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada-cho, Obihiro, Hokkaido, 080-8555, Japan

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Mori Y, Notomi T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother. 2009;15:62–9. You can mount the downlight led ceiling with springs or you can print the downlight holder and use them instead for easier mounting. Lee D, Kim EJ, Kilgore PE, Takahashi H, Ohnishi M, Tomono J, et al. A novel loop-mediated isothermal amplification assay for serogroup identification of Neisseria meningitidis in cerebrospinal fluid. Front Microbiol. 2015;6:1548.

Wu L, van den Hoogen LL, Slater H, Walker PG, Ghani AC, Drakeley CJ, et al. Comparison of diagnostics for the detection of asymptomatic Plasmodium falciparum infections to inform control and elimination strategies. Nature. 2015;528:S86–93. Center for Clinical Infectious Diseases and Research, Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama, 350-0495, Japan J. Zhang, J. Cao, M. Zhu, M. Xu and F. Shi, World J. Microbiol. Biotechnol., 2019, 35, 31 CrossRef PubMed. M. Goto, E. Honda, A. Ogura, A. Nomoto and K.-I. Hanaki, BioTechniques, 2009, 46, 167–172 CrossRef CAS PubMed. Y. D. Han, H.-S. Kim, Y. M. Park, H. J. Chun, J.-H. Kim and H. C. Yoon, ACS Appl. Mater. Interfaces, 2016, 8, 10767–10774 CrossRef CAS PubMed.

Research areas

Since a large proportion of the homogeneous methods (18 out of 23) are based on fluorescence, we first discuss and compare fluorescence-based methods in detail. For this, we extend the general assessment criteria (quantification, multiplexing and detection by naked eye, chapter 4.1) and focus additionally on the modification of primers and probes, the optimization of fluorescence signals and the adaption to different targets. L. A. Rigano, F. Malamud, I. G. Orce, M. P. Filippone, M. R. Marano, A. M. Do Amaral, A. P. Castagnaro and A. A. Vojnov, BMC Microbiol., 2014, 14, 86 CrossRef PubMed. N. Arunrut, J. Kampeera, R. Suebsing and W. Kiatpathomchai, J. Virol. Methods, 2013, 193, 542–547 CrossRef CAS PubMed. Hayashida K, Simukoko H, Simmunza M, Ndebe J, Chota A, Namangala B, et al. Direct detection of falciparum and non-falciparum malaria DNA from a drop of blood with high sensitivity by the dried-LAMP system. Parasit Vectors. 2017;10:26.