She works as a children's bookseller, she has two almost grown up children and she lives in the West Country.

Bursting with period atmosphere and theatrical excitement, this historical murder-mystery from the author of The Boy Who Flew, does NOT disappoint.

This is consistent with previous studies in mouse, chick and human models showing that proliferation drops in the differentiated myocardium of the forming HT, while proliferation remains high in the splanchnic mesoderm ( van den Berg et al.

Here, we established a whole-embryo live-imaging method based on two-photon microscopy that allows whole-tissue tracking at cellular resolution. Ils1 cre/+; Rosa26Rtdtomato +/- embryos at different stages from cc up to heart looping (n = 10) and assessed the appearance of GFP and tdtomato double-positive domains in the HT. B’) Inset in ( B): arrow points the transition between cTnnT+ and cTnnT- domains, corresponding to FHF and SHF, respectively. Elevation of LV end-diastolic pressure (LVEDP) from approximately 5 to 10 mmHg resulted in a twofold increase in average cardiac power [product of LV developed pressure (LVDevP) and CO], whereas myocardial contractility (first derivative of LV pressure, dP/dt) and LVDevP (LV systolic pressure-LVEDP) increased only minimally (5-10%). Once the cc is formed, if cells of the SHF would continuously differentiate, then regions of the forming heart tube contributed by the SHF precursors should appear densely co-labeled with both GFP and tdtomato.

The initial location of this cell population fated to become cc cardiomyocytes delineates a crescent-shaped domain at EHF stage ( Figure 5H). In a second phase, no differentiation occurs while extensive morphogenesis, including splanchnic mesoderm sliding over the endoderm, results in HT formation. To overcome these limitations, we fixed and immunostained embryos against cTnnT after completion of the live-imaging experiments, and imaged them by 3D confocal microscopy.

We constructed a developmental atlas of the mouse cardiovascular system using episcopic fluorescence image capture (EFIC), a histological imaging techniques that provides serial 2D histological images that are high resolution and in complete registration. Regarding the specification of FHF and SHF populations, previous prospective clonal analyses showed that these lineages diverge around gastrulation ( Devine et al. As mentioned above, antero-medial movement of the splanchnic mesoderm can be observed concomitant with the transformation of the transversal HT into the open HT (visible also in Videos 5 and 12). In contrast, a dense tdtomato labeling appears in the GFP-low cells of the splanchnic mesoderm as the cardiac crescent fully differentiates (from t = 2h24 m to t = 4 hr in Figure 8A and C and Video 16).

cre/+ ; Rosa26RmT/mG +/- embryos, in which Cre-recombined cells activate membrane-bound GFP, we could track cells during differentiation, as they transit from a columnar to a round shape and start contracting ( Figure 4G–G’’, Video 7 and bright-field Video 8). Two daughter cells are defined as sharing the same fate if their GFP intensity levels do not differ by more than 1. Takahashi Y, Abe Y, Takashi M, Fujii H, Morisaki A, Nishimura S, Sakon Y, Ito K, Shintani A, Shibata T.